HM Prison Kilmarnock is a prison in Bowhouse, Hurlford near Kilmarnock, East Ayrshire, Scotland. It is situated 3 miles south-east ofHurlford on the Mauchline Road. Its location means it is locally known as Bowhouse Prison.

The prison was opened 25 March 1999 and is privately run by Serco (formerly known as Premier Prison Services) on behalf of the Scottish Prison Service; the first of its kind in Scotland. In 2005 the BBC program Panorama uncovered several failings in the operational running of the prison, resulting in the prison carrying out internal investigations.

The prison, which holds short-term, long-term and remand male adults, currently has 550 inmates.